2D+Time Datasets

Mouse hematopoietic stem cells in hydrogel microwells

Dr. H. Blau, Baxter Laboratory for Stem Cell Biology, Stanford University, USA

Training dataset: http://data.celltrackingchallenge.net/training-datasets/BF-C2DL-HSC.zip✱ (1.6 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/BF-C2DL-HSC.zip (1.6 GB)


Mouse muscle stem cells in hydrogel microwells

Dr. H. Blau, Baxter Laboratory for Stem Cell Biology, Stanford University, USA

Training dataset: http://data.celltrackingchallenge.net/training-datasets/BF-C2DL-MuSC.zip✱ (1.2 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/BF-C2DL-MuSC.zip (1.3 GB)


HeLa cells on a flat glass

Dr. G. van Cappellen. Erasmus Medical Center, Rotterdam, The Netherlands

Training dataset: http://data.celltrackingchallenge.net/training-datasets/DIC-C2DH-HeLa.zip✱ (37 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/DIC-C2DH-HeLa.zip (41 MB)



Human hepatocarcinoma-derived cells expressing the fusion protein YFP-TIA-1

Dr. Alessia Ruggieri and Philipp Klein, Centre for Integrative Infectious Disease Research (CIID), University Hospital Heidelberg, Germany

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C2DL-Huh7.zip (36 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C2DL-Huh7.zip (36 MB)


Rat mesenchymal stem cells on a flat polyacrylamide substrate

Dr. F. Prósper. Cell Therapy laboratory, Center for Applied Medical Research (CIMA), Pamplona, Spain

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C2DL-MSC.zip✱ (72 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C2DL-MSC.zip (71 MB)


GFP-GOWT1 mouse stem cells

Dr. E. Bártová. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N2DH-GOWT1.zip✱ (53 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N2DH-GOWT1.zip (46 MB)


HeLa cells stably expressing H2b-GFP

Mitocheck Consortium

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N2DL-HeLa.zip✱ (182 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N2DL-HeLa.zip (168 MB)


Glioblastoma-astrocytoma U373 cells on a polyacrylamide substrate

Dr. S. Kumar. Department of Bioengineering, University of California at Berkeley, Berkeley CA (USA)

Training dataset: http://data.celltrackingchallenge.net/training-datasets/PhC-C2DH-U373.zip✱ (40 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/PhC-C2DH-U373.zip (38 MB)


Pancreatic stem cells on a polystyrene substrate

Dr. T. Becker and Dr. D. Rapoport. Fraunhofer Institution for Marine Biotechnology, Lübeck, Germany

Training dataset: http://data.celltrackingchallenge.net/training-datasets/PhC-C2DL-PSC.zip✱ (124 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/PhC-C2DL-PSC.zip (106 MB)


Simulated nuclei of HL60 cells stained with Hoescht

Dr. V. Ulman and Dr. D. Svoboda. Centre for Biomedical Image Analysis (CBIA),

Masaryk University, Brno, Czech Republic (Created using MitoGen, part of Cytopacq)

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N2DH-SIM+.zip (91 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N2DH-SIM+.zip (96 MB)


The silver reference segmentation annotations available for a particular training dataset. Such a dataset is included in the primary track of the sixth challenge edition hosted by ISBI 2021.