2D+Time Datasets

ISBI 2013-2015 Datasets

Rat mesenchymal stem cells on a flat polyacrylamide substrate

Dr. F. Prósper. Cell Therapy laboratory, Center for Applied Medical Research (CIMA), Pamplona, Spain

Microscope: PerkinElmer UltraVIEW ERS

Objective lens: Plan-Neofluar 10x/0.3 (Plan-Apo 20x/0.75)

Pixel size (microns): 0.3 x 0.3 (0.3977 x 0.3977)

Time step (min): 20 (30)

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C2DL-MSC.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C2DL-MSC.zip

GFP-GOWT1 mouse stem cells

Dr. E. Bártová. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Microscope: Leica TCS SP5

Objective lens: Plan-Apochromat 63x/1.4 (oil)

Pixel size (microns): 0.240 x 0.240

Time step (min): 5

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N2DH-GOWT1.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N2DH-GOWT1.zip

HeLa cells stably expressing H2b-GFP

Mitocheck Consortium

Microscope: Olympus IX81

Objective lens: Plan 10x/0.4

Pixel size (microns): 0.645 x 0.645

Time step (min): 30

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N2DL-HeLa.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N2DL-HeLa.zip

HeLa cells on a flat glass

Dr. G. van Cappellen. Erasmus Medical Center, Rotterdam, The Netherlands

Microscope: Zeiss LSM 510 Meta

Objective lens: Plan-Apochromat 63x/1.4 (oil)

Pixel size (microns): 0.19 x 0.19

Time step (min): 10

Training dataset: http://data.celltrackingchallenge.net/training-datasets/DIC-C2DH-HeLa.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/DIC-C2DH-HeLa.zip

Glioblastoma-astrocytoma U373 cells on a polyacrylamide substrate

Dr. S. Kumar. Department of Bioengineering, University of California at Berkeley, Berkeley CA (USA)

Microscope: Nikon

Objective lens: Plan Fluor DLL 20x/0.5

Pixel size (microns): 0.65 x 0.65

Time step (min): 15

Training dataset: http://data.celltrackingchallenge.net/training-datasets/PhC-C2DH-U373.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/PhC-C2DH-U373.zip

Pancreatic Stem Cells on a Polystyrene substrate

Dr. T. Becker. Fraunhofer Institution for Marine Biotechnology, Lübeck, Germany

Microscope: Olympus ix-81

Objective lens: UPLFLN 4XPH

Pixel size (microns): 1.6 x 1.6

Time step (min): 10

Training dataset: http://data.celltrackingchallenge.net/training-datasets/PhC-C2DL-PSC.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/PhC-C2DL-PSC.zip

Note: Only frames 150-250 have been used to generate the ground truth for the training data, although the evaluation of the challenge data is based on the entire length -300 frames- of the videos.

Simulated nuclei of HL60 cells stained with Hoescht

Dr. V. Ulman and Dr. D. Svoboda. Centre for Biomedical Image Analysis (CBIA),

Masaryk University, Brno, Czech Republic (Created using MitoGen, part of Cytopacq)

Microscope: Zeiss Axiovert 100S with a Micromax 1300-YHS camera

Objective lens: Plan-Apochromat 40x/1.3 (oil)

Pixel size (microns): 0.125 x 0.125

Time step (min): 29

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N2DH-SIM+.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N2DH-SIM+.zip