3D+Time Datasets

Microscope: PerkinElmer UltraVIEW ERS

Objective lens: Plan-Apochromat 63x/1.2 (water)

Voxel size (microns): 0.126 x 0.126 x 1.0

Time step (min): 2

Additional information: IEEE Transactions on Medical Imaging, 2019

Microscope: PerkinElmer UltraVIEW ERS

Objective lens: Plan-Apochromat 63x/1.2 (water)

Voxel size (microns): 0.126 x 0.126 x 0.5

Time step (min): 2 (1)

Additional information: IEEE Transactions on Medical Imaging, 2013

Microscope: Olympus FluoView F1000

Objective lens: Plan 20x/0.7

Voxel size (microns): 1.242 x 1.242 x 6.0

Time step (min): 80

Microscope: Zeiss LSM 510 Meta

Objective lens: Plan-Apochromat 63x/1.4 (oil)

Voxel size (microns): 0.09 x 0.09 x 1.0

Time step (min): 1 (1.5)

Additional information: Nature Methods, 2008

Microscope: Zeiss LSM 510

Objective lens: Plan-Apochromat 63x/1.4 (oil)

Voxel size (microns): 0.202 x 0.202 x 1.0

Time step (min): 9.5

Additional information: IEEE Transactions on Medical Imaging, 2010

Microscope: SIMView light-sheet microscope

Objective lens: 16x/0.8 (water)

Voxel size (microns): 0.406 x 0.406 x 2.03

Time step (sec): 30

Additional information: Nature Methods, 2014

Only the cells that form the developing nervous system will be used for the evaluation of the detection, segmentation, and tracking accuracy. Those cells can be identified in the first frame of the provided gold reference tracking annotation. Be aware that any other cells segmented and tracked will be considered as errors by the evaluation software used for the Cell Tracking Benchmark. By contrast, all extra cells detected and segmented will automatically be filtered out by the evaluation software used for the Cell Segmentation Benchmark, and thus will not be penalized at all.

Microscope: Zeiss LightSheet LZ.1

Objective lens: Plan-Apochromat 20x/1.0 (water)

Voxel size (microns): NA due to cartographic projections

Time step (min): 1.5

Only the lineages of cells in the blastoderm of the beetle, which are at the border of the embryonic and extra embryonic tissues will be used for the evaluation of the detection, segmentation, and tracking accuracy. Those cells can be identified in the first frame of the provided gold reference tracking annotation. Be aware that any other cells segmented and tracked will be considered as errors by the evaluation software used for the Cell Tracking Benchmark. By contrast, all extra cells detected and segmented will automatically be filtered out by the evaluation software used for the Cell Segmentation Benchmark, and thus will not be penalized at all.

Microscope: Zeiss LightSheet LZ.1

Objective lens: Plan-Apochromat 20x/1.0 (water)

Voxel size (microns): 0.38 x 0.38 x 0.38

Time step (min): 1.5

Only the lineages of cells in the blastoderm of the beetle, which are at the border of the embryonic and extra embryonic tissues will be used for the evaluation of the detection, segmentation, and tracking accuracy. Those cells can be identified in the first frame of the provided gold reference tracking annotation. Be aware that any other cells segmented and tracked will be considered as errors by the evaluation software used for the Cell Tracking Benchmark. By contrast, all extra cells detected and segmented will automatically be filtered out by the evaluation software used for the Cell Segmentation Benchmark, and thus will not be penalized at all. Note that the image data was acquired using a light-sheet microscope and fused from multiple viewpoints. Some views may not be registered perfectly (resulting in display of clearly false, outstanding nuclei) or the views altogether do not have to cover the full volume (resulting in an artificial localized dark patch that stretches along one image axis). The cells to be tracked were chosen so that their lineages avoid the regions containing these artifacts.

Microscope: PerkinElmer UltraVIEW ERS

Objective lens: Plan-Apochromat 63x/1.2 (water)

Voxel size (microns): 0.126 x 0.126 x 1.0

Time step (sec): 20

Additional information: IEEE Transactions on Medical Imaging, 2018

Microscope: Zeiss Axiovert 100S with a Micromax 1300-YHS camera

Objective lens: Plan-Apochromat 40x/1.3 (oil)

Voxel size (microns): 0.125 x 0.125 x 0.200

Time step (min): 29

Additional information: IEEE Transactions on Medical Imaging, 2016