3D+Time Datasets

GFP-transfected H157 Lung Cancer cells embedded in a matrigel matrix

Dr. A. Rouzaut. Cell Adhesion and Metastasis Laboratory, Center for Applied Medical Research (CIMA). Pamplona. Spain

Microscope: PerkinElmer UltraVIEW ERS

Objective lens: Plan-Apochromat 63x/1.2 Water

Voxel size (microns): 0.126 x 0.126 x 0.5

Time step (min): 2 (1)

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C3DH-H157.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C3DH-H157.zip

MDA231 human breast carcinoma cells infected with a pMSCV vector including the GFP sequence, embedded in a collagen matrix

Dr. Roger Kamm, Dept. of Biological Engineering, Massachusetts Institute of Technology. Cambridge MA (USA)

Microscope: Olympus FluoView F1000

Objective lens: Plan 20x/0.7

Voxel size (microns): 1.242 x 1.242 x 6.0

Time step (min): 80

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C3DL-MDA231.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C3DL-MDA231.zip

Chinese Hamster Ovarian (CHO) nuclei overexpressing GFP-PCNA

Dr. J. Essers. Dept. of Cell Biology, Erasmus Medical Center. Rotterdam. The Netherlands

Microscope: Zeiss LSM 510

Objective lens: Plan-Apochromat 63x/1.4 Oil

Voxel size (microns): 0.202 x 0.202 x 1.0

Time step (min): 9.5

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DH-CHO.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DH-CHO.zip

C.elegans developing embryo

Waterston Lab, The George Washington University. Washington DC (USA)

Microscope: Zeiss LSM 510 Meta

Objective lens: Plan-Apochromat 63X/1.4 (oil)

Pixel size (microns): 0.09 x 0.09 x 1.0

Time step (min): 1 or 1.5

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DH-CE.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DH-CE.zip

Developing Drosophila Melanogaster embryo

Dr. Philipp Keller, Howard Hughes Medical Institute, Janelia Farms Research Campus, Ashburn VA (USA)

Microscope: SIMView light-sheet microscope

Objective lens: 16X/0.8 (water)

Pixel size (microns): 0.406 x 0.406 x 2.03

Time step (sec): 30

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DL-DRO.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DL-DRO.zip

Note: Only the cells that form the developing nervous system will be used for the evaluation of the segmentation and tracking accuracy. Those cells can be identified in the first frame of the provided tracking ground truth (TRA GT). Be aware that any other cells segmented and tracked will be considered as errors by the evaluation software.

Simulated nuclei of HL60 cells stained with Hoescht

Dr. V.Ulman and Dr. D. Svoboda. Centre for Biomedical Image Analysis (CBIA).

Masaryk University. Brno. Czech Republic (Created using MitoGen, part of Cytopacq)

Microscope: Zeiss Axiovert 100S with a Micromax 1300-YHS camera

Objective lens: Plan-Apochromat 40X/1.3 (oil)

Pixel size (microns): 0.125 x 0.125 x 0.200

Time step (min): 29

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DH-SIM+.zip

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DH-SIM+.zip