3D+Time Datasets

GFP-actin-stained A549 Lung Cancer cells embedded in a Matrigel matrix

Dr. A. Rouzaut. Cell Adhesion and Metastasis Laboratory, Center for Applied Medical Research (CIMA), Pamplona, Spain

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C3DH-A549.zip✱ (244 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C3DH-A549.zip (294 MB)


GFP-transfected H157 Lung Cancer cells embedded in a matrigel matrix

Dr. A. Rouzaut. Cell Adhesion and Metastasis Laboratory, Center for Applied Medical Research (CIMA), Pamplona, Spain

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C3DH-H157.zip✱ (7.0 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C3DH-H157.zip (7.1 GB)


MDA231 human breast carcinoma cells infected with a pMSCV vector including the GFP sequence, embedded in a collagen matrix

Dr. R. Kamm. Dept. of Biological Engineering, Massachusetts Institute of Technology, Cambridge MA (USA)

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C3DL-MDA231.zip✱ (182 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C3DL-MDA231.zip (179 MB)


C.elegans developing embryo

Waterston Lab, University of Washington, Seattle, WA, USA

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DH-CE.zip✱ (3.1 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DH-CE.zip (1.7 GB)


Chinese Hamster Ovarian (CHO) nuclei overexpressing GFP-PCNA

Dr. J. Essers. Dept. of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DH-CHO.zip✱ (98 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DH-CHO.zip (105 MB)


Developing Drosophila Melanogaster embryo

Dr. P. Keller. Howard Hughes Medical Institute, Janelia Farms Research Campus, Ashburn VA, USA

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DL-DRO.zip (5.8 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DL-DRO.zip (5.9 GB)


Developing Tribolium Castaneum embryo (3D cartographic projection)

Dr. A. Jain. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DL-TRIC.zip (20.6 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DL-TRIC.zip (19.9 GB)


Developing Tribolium Castaneum embryo

Dr. A. Jain. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DL-TRIF.zip (320 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DL-TRIF.zip (467 GB)


Simulated GFP-actin-stained A549 Lung Cancer cells embedded in a Matrigel matrix

Dr. M. Maška and Dr. D. V. Sorokin. Centre for Biomedical Image Analysis (CBIA),

Masaryk University, Brno, Czech Republic (Created using FiloGen, part of Cytopacq)

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-C3DH-A549-SIM.zip (314 MB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-C3DH-A549-SIM.zip (327 MB)


Simulated nuclei of HL60 cells stained with Hoechst

Dr. V. Ulman and Dr. D. Svoboda. Centre for Biomedical Image Analysis (CBIA),

Masaryk University, Brno, Czech Republic (Created using MitoGen, part of Cytopacq)

Training dataset: http://data.celltrackingchallenge.net/training-datasets/Fluo-N3DH-SIM+.zip (3.1 GB)

Challenge dataset: http://data.celltrackingchallenge.net/challenge-datasets/Fluo-N3DH-SIM+.zip (5.9 GB)


The silver reference segmentation annotations available for a particular training dataset. Such a dataset is included in the primary track of the sixth challenge edition hosted by ISBI 2021.