Dataset Description

The datasets consist of 2D and 3D time-lapse video sequences of fluorescent counterstained nuclei or cells moving on top or immersed in a substrate, along with 2D Phase Contrast and Differential Interference Contrast (DIC) microscopy videos of cells moving on a flat substrate. The videos cover a wide range of cell types and quality (spatial and temporal resolution, noise levels etc.) In addition, we provide 2D and 3D videos of synthetic fluorescently stained cells and nuclei of different shapes and motion patterns.

All 2D+t and 3D+t datasets are available for download to anyone. Please check carefully the “Conditions of use of the images” at the end of this page before downloading any of these datasets. For technical support, please contact Martin Maška (xmaska@fi.muni.cz).

The ground truth, consisting of manually annotated videos (segmentation) and acyclic graphs (detection and tracking), was generated following the annotation guidelines described in the following document:

 Annotation procedure.pdf

and both the original and ground truth files were named and created following the conventions described in:

 Naming and file content conventions.pdf

 

Conditions of use of the images:

1) Please, include a reference to these Bioinformatics and Nature Methods papers in any publication resulting from the use of any of the DIC-C2DH-HeLa, Fluo-C2DL-MSC, Fluo-C3DH-H157, Fluo-C3DL-MDA231, Fluo-N2DH-GOWT1, Fluo-N2DL-HeLa, Fluo-N3DH-CE, Fluo-N3DH-CHO, Fluo-N3DL-DRO, PhC-C2DH-U373, PhC-C2DL-PSC, Fluo-N2DH-SIM+, and Fluo-N3DH-SIM+ datasets.

2) The use of any of the Fluo-C3DH-A549, Fluo-N3DL-TRIC, and Fluo-C3DH-A549-SIM datasets in a publication is generally possible, but only with the explicit consent given by the challenge organizers and the particular dataset provider.

3) The use of any of the datasets for other than research purposes is generally possible, but only with the explicit consent given by the challenge organizers and the particular dataset provider.